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Full length native protein from human erythrocytes.
Our Abpromise guarantee covers the use of ab9385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Milk blocking may cause 'no bands' problem. Please try BSA as well.|
Lane 1 - 4 : GAPDH antibody - Loading Control (ab9385) at 1 ug/ml
Lane 1 : HeLa cell lysate at 20 ug
Lane 2 : A431 cell lysate at 20 ug
Lane 3 : Jurkat cell lysate at 20 ug
Lane 4 : 293 cell lysate at 20 ug
Performed under reducing conditions.
Exposure time : 1 minute