GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482)

Product Name 

GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (HRP)

Immunogen 

Full length native protein from human erythrocytes.

Reacts with 
(species key)

Hu, Ms, Rat, Chk, ChHm, Cow, Dog, Pig, Rb, Xl

Specificity 

This antibody shows a single clean band in Western blotting on cell lysates.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/5000-1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa).

NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody following concerning customer feedback on the lack of signal with some of the vials. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images of our tests available on the datasheet of the non-HRP conjugated version of the antibody, ab9484). This change in the characteristics of the antibody is due to a recent update to the production process.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Isotype/Loading Controls >> Loading Controls >> GAPDH
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease

Relevance 

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam 9484

Myeloma 

Sp2/0-Ag14

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Protein A purified

Conjugation notes 

This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, PBS

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

See below for GAPDH antibody [mAbcam 9484] (HRP) - Loading Control images, references, products related to ab9482 and other tools.

GAPDH antibody [mAbcam 9484] (HRP) - Loading Control images:

References for GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482)

  This product has been used in: (two most recent references)

Silveira AC et al. Over-expression of the BRMS1 family member SUDS3 does not suppress metastasis of human cancer cells. Cancer Lett 276:32-7 (2009). WB; Human. PubMed: 19070953

Chen YF et al. Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice. Genes Dev 23:1183-94 (2009). WB; Mouse. PubMed: 19451219

See all 18 publication references for this product.


If you publish research using ab9482 please let us know so that we can cite the reference on this datasheet.

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