Full length native protein from human erythrocytes.
According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.
Alternative versions available:
Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
Anti-GAPDH antibody (Alexa Fluor® 647) [mAbcam 9484] (ab196697)
Anti-GAPDH antibody (Alexa Fluor® 680) [mAbcam 9484] (ab184095)
Anti-GAPDH antibody (Alexa Fluor® 790) [mAbcam 9484] (ab184578)
This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.
Our Abpromise guarantee covers the use of ab9482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa). NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process.|