Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482)

Overview

  • Product nameAnti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)
    See all GAPDH primary antibodies
  • Description
    Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (HRP)
  • ConjugationHRP
  • Conjugation notesThis antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.
  • SpecificityThis antibody shows a single clean band in Western blotting on cell lysates.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Xenopus laevis, Chinese Hamster
  • Immunogen

    Full length native protein from human erythrocytes.

  • General notes

    According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

Applications

Our Abpromise guarantee covers the use of ab9482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa). NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process.

Target

Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) images

  • Lanes 1 - 4 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482) at 1/5000 dilution ((Blocked in 5% MILK))
    Lanes 5 - 8 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482) at 1/5000 dilution ((Blocked in 5% BSA))

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40.2 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482)

This product has been referenced in:
  • Chagniel L  et al. Regulation of tyrosine phosphatase STEP61 by protein kinase A during motor skill learning in mice. PLoS One 9:e86988 (2014). WB . Read more (PubMed: 24466306) »
  • Zins K  et al. Egr-1 upregulates Siva-1 expression and induces cardiac fibroblast apoptosis. Int J Mol Sci 15:1538-53 (2014). WB . Read more (PubMed: 24451137) »

See all 37 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to hear of the trouble you are having with lot 937205 in ChIP. I looked in our records and there was one other complaint of a similar nature regarding ChIP with this lot of ab21873. We do have a new lot in stock n...

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Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (12% polyacrylamide)
Sample Human Cell lysate - whole cell (Huh-7.5 liver cells)
Specification Huh-7.5 liver cells
Treatment siRNA treated cells + untreated control lysate
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Rat Tissue lysate - other (Cytosolic fractions from skeletal muscles)
Specification Cytosolic fractions from skeletal muscles
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 31 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Skeletal muscle)
Loading amount 20 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted May 14 2013

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab9482.

To check the status of the order please contact ou...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for contacting us and describing the issue you are having with ab9482.

I have issued a free of charge replacement from a different lot. To check the status of the order please contact our Customer Service team and reference this nu...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen.


In der Tat sieht es so aus, als ob der Antikörper unter diesen Bedingungen funktioniert haben müsste. Zwar bekommen Sie ein Signal, aber es ist in der Tat viel zu schwa...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Loading amount 50 µg
Specification Heart
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Mar 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"