Overview

  • Product name
    Anti-GAPDHS antibody
  • Description
    Rabbit polyclonal to GAPDHS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 161-391 of Human GAPDHS.

  • Positive control
    • NT2D1, IMR32, U87-MG, MCF7 and NIH3T3 cell lysates; MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
  • Tissue specificity
    Testis specific.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities
    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Domain
    The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • G3PT_HUMAN antibody
    • GAPD2 antibody
    • GAPDH 2 antibody
    • GAPDH-2 antibody
    • GAPDH2 antibody
    • GAPDHS antibody
    • GAPDS antibody
    • Glyceraldehyde 3 phosphate dehydrogenase spermatogenic antibody
    • Glyceraldehyde 3 phosphate dehydrogenase, testis specific antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HSD 35 antibody
    • HSD35 antibody
    • Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2 antibody
    • Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 antibody
    • Spermatogenic glyceraldehyde 3 phosphate dehydrogenase antibody
    • Spermatogenic glyceraldehyde-3-phosphate dehydrogenase antibody
    • testis-specific antibody
    see all

Images

  • Anti-GAPDHS antibody (ab153802) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Anti-GAPDHS antibody (ab153802) at 1/5000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed MCF7 cells labeling GAPDHS with ab153802 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Zhang J  et al. Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression. Mol Cancer 16:151 (2017). Read more (PubMed: 28893265) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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