The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml. IHC-P: Use at a concentration of 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
Tissue specificityTestis specific.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
DomainThe testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components.
GAPDHS antibody (ab57062) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human testis.
Western blot - GAPDHS antibody (ab57062)
Western blot against tagged recombinant protein immunogen using ab57062 GAPDHS antibody at 1ug/ml. Predicted band size of immunogen is 71 kDa
References for Anti-GAPDHS antibody (ab57062)
This product has been referenced in:
Huang ZJ et al. Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells. Mol Med Rep11:1647-54 (2015).
Read more (PubMed: 25405855) »