Overview

  • Product nameAnti-GAPex 5 antibody
    See all GAPex 5 primary antibodies
  • Description
    Rabbit polyclonal to GAPex 5
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • Immunogen

    A synthetic peptide corresponding to a region between residue 550 and 600 of human GAPex 5 (BAG10470.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 165 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceGAPex 5 plays a role in several processes such as endocytosis, insulin receptor internalisation and LC2A4/GLUT4 trafficking. It acts as both a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF). GAPex 5 is a GEF for the small G protein Rab31, promoting the exchange of GDP bound to Rab31 for GTP and thus regulating LC2A4/GLUT4 trafficking. In the absence of insulin, GAPex 5 maintains Rab31 in an active (GTP-bound) state, thereby promoting a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, keeping LC2A4/GLUT4 inside the cells. Following insulin stimulation, GAPex 5 is translocated to the plasma membrane, enabling the release of LC2A4/GLUT4 from the intracellular storage vesicles. GAPex 5 is also involved in EGFR trafficking and degradation, and also has GEF activity for Rab5 and GAP activity for Ras.
    • Cellular localizationCell Membrane (peripheral membrane protein) and Cytoplasmic
    • Database links
    • Alternative names
      • GAPEX5 antibody
      • GAPVD1 antibody
      • GTPase activating protein and VPS9 domains 1 antibody
      • KIAA1521 antibody
      • Rab5 activating protein 6 antibody
      • RAP6 antibody
      see all

    Anti-GAPex 5 antibody images

    • All lanes : Anti-GAPex 5 antibody (ab86033) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 165 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • ab86033, at 0.1 µg/ml, staining GAPex 5 in HeLa whole cell lysate immunoprecipitated using ab86033 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: by chemiluminescence with an exposure time of 30 seconds.

    References for Anti-GAPex 5 antibody (ab86033)

    ab86033 has not yet been referenced specifically in any publications.

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