• Product name
  • Description
    Rabbit polyclonal to GAPex 5
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    A synthetic peptide corresponding to a region between residue 550 and 600 of human GAPex 5 (BAG10470.1).

  • Positive control
    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab86033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 165 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      GAPex 5 plays a role in several processes such as endocytosis, insulin receptor internalisation and LC2A4/GLUT4 trafficking. It acts as both a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF). GAPex 5 is a GEF for the small G protein Rab31, promoting the exchange of GDP bound to Rab31 for GTP and thus regulating LC2A4/GLUT4 trafficking. In the absence of insulin, GAPex 5 maintains Rab31 in an active (GTP-bound) state, thereby promoting a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, keeping LC2A4/GLUT4 inside the cells. Following insulin stimulation, GAPex 5 is translocated to the plasma membrane, enabling the release of LC2A4/GLUT4 from the intracellular storage vesicles. GAPex 5 is also involved in EGFR trafficking and degradation, and also has GEF activity for Rab5 and GAP activity for Ras.
    • Cellular localization
      Cell Membrane (peripheral membrane protein) and Cytoplasmic
    • Database links
    • Alternative names
      • GAPEX5 antibody
      • GAPVD1 antibody
      • GTPase activating protein and VPS9 domains 1 antibody
      • KIAA1521 antibody
      • Rab5 activating protein 6 antibody
      • RAP6 antibody
      see all

    Anti-GAPex 5 antibody images

    • All lanes : Anti-GAPex 5 antibody (ab86033) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 165 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • ab86033, at 0.1 µg/ml, staining GAPex 5 in HeLa whole cell lysate immunoprecipitated using ab86033 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: by chemiluminescence with an exposure time of 30 seconds.

    References for Anti-GAPex 5 antibody (ab86033)

    ab86033 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86033.
    Please use the links above to contact us or submit feedback about this product.


    Sign up