Recombinant
RabMAb

Anti-GARNL1 antibody [EPR13592] (ab182148)

Overview

  • Product name
    Anti-GARNL1 antibody [EPR13592]
    See all GARNL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13592] to GARNL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GARNL1 aa 650-800. The exact sequence is proprietary.
    Database link: Q6GYQ0

  • Positive control
    • 293 and HeLa cell lysates; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab182148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 230 kDa).
ICC/IF 1/100.

Target

  • Relevance
    GTPase activating Rap/RanGAP domain-like 1 (GARNL1) is an interacting partner of the transcription factor TCF3/isoform E12, mainly in the developing embryonic forebrain. It may be an important transcriptional regulator of downstream target genes under the control of TCF3/E12, by disrupting HLH dimer formation of TCF3/E12 with other proteins and may be involved in neuronal differentiation.
  • Cellular localization
    Cytoplasmic and Nuclear. Translocated to the nucleus when associated with TCF3/E12
  • Database links
  • Alternative names
    • DKFZp566D133 antibody
    • DKFZp667F074 antibody
    • GAP related interacting partner to E12 antibody
    • GAP related interacting protein to E12 antibody
    • GARN L1 antibody
    • GARNL 1 antibody
    • GRIPE antibody
    • GTPase activating RANGAP domain like 1 antibody
    • GTPase activating Rap/RanGAP domain like 1 antibody
    • KIAA0884 antibody
    • p240 antibody
    • Ral GTPase activating protein catalytic alpha subunit 1 antibody
    • Ral GTPase activating protein subunit alpha 1 antibody
    • Ral GTPase activating protein, alpha subunit 1 (catalytic) antibody
    • RalGAPalpha1 antibody
    • Tuberin like protein 1 antibody
    • TULIP 1 antibody
    • TULIP1 antibody
    see all

Images

  • Anti-GARNL1 antibody [EPR13592] (ab182148) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 230 kDa

  • Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling GARNL1 with ab182148 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 diution and counterstained with DAPI.

  • Anti-GARNL1 antibody [EPR13592] (ab182148) at 1/10000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 230 kDa

References

ab182148 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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