The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 230 kDa).
GTPase activating Rap/RanGAP domain-like 1 (GARNL1) is an interacting partner of the transcription factor TCF3/isoform E12, mainly in the developing embryonic forebrain. It may be an important transcriptional regulator of downstream target genes under the control of TCF3/E12, by disrupting HLH dimer formation of TCF3/E12 with other proteins and may be involved in neuronal differentiation.
Cytoplasmic and Nuclear. Translocated to the nucleus when associated with TCF3/E12
Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling GARNL1 with ab182148 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 diution and counterstained with DAPI.
Western blot - Anti-GARNL1 [EPR13592] antibody (ab182148)
Anti-GARNL1 antibody [EPR13592] (ab182148) at 1/10000 dilution + 293 cell lysate at 20 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution