Anti-GARS antibody [EPR7157] (ab125008)

Overview

  • Product nameAnti-GARS antibody [EPR7157]
    See all GARS primary antibodies
  • Description
    Rabbit monoclonal [EPR7157] to GARS
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide, corresponding to residues near the C-terminus of Human GARS.

  • Positive control
    • Jurkat, HeLa, U87-MG, 293T, C6, RAW 264.7, and Neuro-2a cell lysates; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR7157
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    Anti-GARS antibody [EPR7157] images

    • All lanes : Anti-GARS antibody [EPR7157] (ab125008) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : RAW 264.7 cell lysate
      Lane 7 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 83 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab125008 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.

    References for Anti-GARS antibody [EPR7157] (ab125008)

    ab125008 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Apr 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"