Recombinant
RabMAb

Anti-GART antibody [EPR11622] (ab169550)

Overview

  • Product name
    Anti-GART antibody [EPR11622]
    See all GART primary antibodies
  • Description
    Rabbit monoclonal [EPR11622] to GART
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human GART (UniProt P22102).

  • Positive control
    • HeLa, HepG2, K562 and A431 cell lysates; Human colonic adenocarcinoma and tonsil tissues; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 107 kDa.
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Pathway
      Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.
      Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
      Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
    • Sequence similarities
      In the N-terminal section; belongs to the GARS family.
      In the central section; belongs to the AIR synthase family.
      In the C-terminal section; belongs to the GART family.
      Contains 1 ATP-grasp domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5'-phosphoribosylglycinamide transformylase antibody
      • AIR synthase antibody
      • AIRS antibody
      • GAR transformylase antibody
      • GARS antibody
      • GART antibody
      • GARTF antibody
      • Glycinamide ribonucleotide synthetase antibody
      • MGC47764 antibody
      • PAIS antibody
      • PGFT antibody
      • Phosphoribosyl-aminoimidazole synthetase antibody
      • Phosphoribosylglycinamide formyltransferase antibody
      • Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase antibody
      • Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29 antibody
      • Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase antibody
      • Phosphoribosylglycinamide synthetase antibody
      • PRGS antibody
      • PUR2_HUMAN antibody
      • Trifunctional purine biosynthetic protein adenosine 3 antibody
      see all

    Images

    • All lanes : Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 107 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling GART with ab169550 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GART with ab169550 at 1/50 dilution.
    • ab169550 staining GART in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      See Abreview

    • Immunofluorescent analysis of HepG2 cells labeling GART with ab169550 at 1/100 dilution.
    • Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 107 kDa

    References

    ab169550 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 23 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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