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Rabbit polyclonal to Gas1
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Gas1.
(Peptide available as
This antibody gave a positive signal in HEK293 whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
Use a concentration of 5 µg/ml.
Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.
Information by UniProt
Western blot - Gas1 antibody (ab58653)
Anti-Gas1 antibody (ab58653) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Predicted band size:
Observed band size:
37 kDa (
why is the actual band size different from the predicted?
Additional bands at:
23 kDa. We are unsure as to the identity of these extra bands.
Growth arrest-specific protein 1 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - Gas1 antibody (ab58653)
ICC/IF image of ab58653 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58653, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.
has not yet been referenced specifically in any publications.
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