This antibody gave a positive signal in Human and Mouse Kidney tissue lysate as well as both HEK293 and HepG2 whole cell lysate.
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Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
Belongs to the GAS2 family. Contains 1 CH (calponin-homology) domain. Contains 1 GAR domain.
Specifically expressed at growth arrest.
Cleaved, during apoptosis, on a specific aspartic residue by caspases. Phosphorylated on serine residues during the G0-G1 transition phase.
Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
All lanes : Anti-GAS2 antibody (ab106505) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : Human kidney tissue lysate - total protein (ab30203) Lane 3 : Kidney (Mouse) Tissue Lysate Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa Observed band size: 35 kDa Additional bands at: 73 kDa, 89 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
In Human adult liver, lung, kidney and fetal liver tissue lysates we see a much stronger band at 73kDa. Abcam are currently unsure about the identity of this band and would welcome feedback from customers working on this protein.