This antibody gave a positive signal in Human and Mouse Kidney tissue lysate as well as both HEK293 and HepG2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
FunctionMay play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
Tissue specificityUbiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
Sequence similaritiesBelongs to the GAS2 family. Contains 1 CH (calponin-homology) domain. Contains 1 GAR domain.
Developmental stageSpecifically expressed at growth arrest.
Post-translational modificationsCleaved, during apoptosis, on a specific aspartic residue by caspases. Phosphorylated on serine residues during the G0-G1 transition phase.
Cellular localizationCytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.