Overview

  • Product name
    Anti-GAS2 antibody [EPR1742(2)]
    See all GAS2 primary antibodies
  • Description
    Rabbit monoclonal [EPR1742(2)] to GAS2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N-terminus in Human GAS2.

  • Positive control
    • HepG2, fetal liver, fetal kidney, and Jurkat lysates, Human stomach tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
    • Tissue specificity
      Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
    • Sequence similarities
      Belongs to the GAS2 family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 GAR domain.
    • Developmental stage
      Specifically expressed at growth arrest.
    • Post-translational
      modifications
      Cleaved, during apoptosis, on a specific aspartic residue by caspases.
      Phosphorylated on serine residues during the G0-G1 transition phase.
    • Cellular localization
      Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
    • Information by UniProt
    • Database links
    • Alternative names
      • GAS 2 antibody
      • GAS-2 antibody
      • Gas2 antibody
      • GAS2_HUMAN antibody
      • Growth arrest specific 2 antibody
      • Growth arrest specific protein 2 antibody
      • Growth arrest-specific protein 2 antibody
      • MGC32610 antibody
      see all

    Images

    • All lanes : Anti-GAS2 antibody [EPR1742(2)] (ab109762) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
      Observed band size : 35 kDa
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab109762 at a dilution of 1/50.

    References

    This product has been referenced in:
    • Sun L  et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). Read more (PubMed: 26358320) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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