The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
1/10 - 1/100.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notesIs unsuitable for Flow Cyt or ICC.
FunctionMay play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
Tissue specificityUbiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
Sequence similaritiesBelongs to the GAS2 family. Contains 1 CH (calponin-homology) domain. Contains 1 GAR domain.
Developmental stageSpecifically expressed at growth arrest.
Post-translational modificationsCleaved, during apoptosis, on a specific aspartic residue by caspases. Phosphorylated on serine residues during the G0-G1 transition phase.
Cellular localizationCytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.