Anti-Gastrin antibody (ab16035)
Key features and details
- Rabbit polyclonal to Gastrin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Gastrin antibody
See all Gastrin primary antibodies -
Description
Rabbit polyclonal to Gastrin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Dog -
Immunogen
Synthetic peptide within Human Gastrin aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P01350 -
Positive control
- Pituitary or stomach
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General notes
This antibody may be used to study the differential tissue expression and intracellular and subcellular localization of gastrin in neuroendocrine cells of the gastrointestinal tract, and in the CNS. Antibodies to gastrin are also useful for the identificationand detection of gastrin in normal and neoplastic tissue.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
This antibody may be used to study the differential tissue expression and intracellular and subcellular localization of gastrin in neuroendocrine cells of the gastrointestinal tract,and in the CNS. Antibodies to gastrin are also useful for the identificationand detection of gastrin in normal and neoplastic tissue. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200.
|
Notes |
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IHC-P
1/200. |
Target
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Function
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. -
Sequence similarities
Belongs to the gastrin/cholecystokinin family. -
Post-translational
modificationsTwo different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2520 Human
- Omim: 137250 Human
- SwissProt: P01353 Dog
- SwissProt: P55885 Horse
- SwissProt: P01350 Human
- Unigene: 2681 Human
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Alternative names
- Component II antibody
- G14 antibody
- G17 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab16035 has been referenced in 1 publication.
- Lennerz JK et al. The Transcription Factor MIST1 Is a Novel Human Gastric Chief Cell Marker Whose Expression Is Lost in Metaplasia, Dysplasia, and Carcinoma. Am J Pathol 177:1514-33 (2010). IHC-P ; Human . PubMed: 20709804