Overview

  • Product nameAnti-GATA1 antibody
    See all GATA1 primary antibodies
  • Description
    Rabbit polyclonal to GATA1
  • SpecificityThis antibody is specific for GATA1.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized non-phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 310.

  • Positive control
    • Extracts from COS7 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionTranscriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
  • Tissue specificityErythrocytes.
  • Involvement in diseaseDefects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • DomainThe two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
  • Post-translational
    modifications
    Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anemia, X-linked, without thrombocytopenia, included antibody
    • ERYF 1 antibody
    • Eryf1 antibody
    • Erythroid transcription factor antibody
    • Erythrold transcription factor 1 antibody
    • GATA 1 antibody
    • GATA binding factor 1 antibody
    • GATA binding protein 1 (globin transcription factor 1) antibody
    • GATA binding protein 1 antibody
    • GATA-1 antibody
    • GATA-binding factor 1 antibody
    • GATA1 antibody
    • GATA1_HUMAN antibody
    • GF 1 antibody
    • GF-1 antibody
    • GF1 antibody
    • Globin transcription factor 1 antibody
    • NF E1 antibody
    • NF E1 DNA binding protein antibody
    • NF-E1 DNA-binding protein antibody
    • NFE 1 antibody
    • NFE1 antibody
    • Nuclear factor erythroid 1 antibody
    • Transcription factor GATA1 antibody
    • XLANP antibody
    • XLTDA antibody
    • XLTT antibody
    see all

Anti-GATA1 antibody images

  • All lanes : Anti-GATA1 antibody (ab47490) at 1/500 dilution

    Lane 1 : extracts from COS7 cells, untreated
    Lane 2 : extracts from COS7 cells with synthesized peptide at 1 µg/ml

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 43 kDa

References for Anti-GATA1 antibody (ab47490)

This product has been referenced in:
  • Sankaran VG  et al. Cyclin D3 coordinates the cell cycle during differentiation to regulate erythrocyte size and number. Genes Dev 26:2075-87 (2012). Read more (PubMed: 22929040) »
  • Xu J  et al. Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6. Genes Dev 24:783-98 (2010). WB, ChIP/Chip ; Human . Read more (PubMed: 20395365) »

See all 2 Publications for this product

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