• Product nameAnti-GATA1 antibody
    See all GATA1 primary antibodies
  • Description
    Rabbit polyclonal to GATA1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GATA1.

    (Peptide available as ab94381.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human bone marrow; Human spleen; Human liver.



Our Abpromise guarantee covers the use of ab85466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
    • Tissue specificityErythrocytes.
    • Involvement in diseaseDefects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
      Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
      Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
    • Sequence similaritiesContains 2 GATA-type zinc fingers.
    • DomainThe two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
    • Post-translational
      Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
      Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anemia, X-linked, without thrombocytopenia, included antibody
      • ERYF 1 antibody
      • Eryf1 antibody
      • Erythroid transcription factor antibody
      • Erythrold transcription factor 1 antibody
      • GATA 1 antibody
      • GATA binding factor 1 antibody
      • GATA binding protein 1 (globin transcription factor 1) antibody
      • GATA binding protein 1 antibody
      • GATA-1 antibody
      • GATA-binding factor 1 antibody
      • GATA1 antibody
      • GATA1_HUMAN antibody
      • GF 1 antibody
      • GF-1 antibody
      • GF1 antibody
      • Globin transcription factor 1 antibody
      • NF E1 antibody
      • NF E1 DNA binding protein antibody
      • NF-E1 DNA-binding protein antibody
      • NFE 1 antibody
      • NFE1 antibody
      • Nuclear factor erythroid 1 antibody
      • Transcription factor GATA1 antibody
      • XLANP antibody
      • XLTDA antibody
      • XLTT antibody
      see all

    Anti-GATA1 antibody images

    • All lanes : Anti-GATA1 antibody (ab85466) at 1 µg/ml

      Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
      Lane 2 : Human spleen tissue lysate - total protein (ab29699)
      Lane 3 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes

    References for Anti-GATA1 antibody (ab85466)

    ab85466 has not yet been referenced specifically in any publications.

    Product Wall

    Thanks for your enquiry.

    ab85466 may be useful for ChIP or IP, although as you note it has not been tested in either or these applications. And, since the sequence of C. elegans is nearly identical to aa 250 - 350 of the human sequence it is ...

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