Anti-GATA1 (phospho S142) antibody (ab28816)


  • Product nameAnti-GATA1 (phospho S142) antibody
    See all GATA1 primary antibodies
  • Description
    Rabbit polyclonal to GATA1 (phospho S142)
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human GATA1 around the phosphorylation site of Serine 142.

  • Positive control
    • k562 cell lysate


Associated products


Our Abpromise guarantee covers the use of ab28816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa.
ELISA 1/4000.
IP Use at an assay dependent concentration.


  • FunctionTranscriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
  • Tissue specificityErythrocytes.
  • Involvement in diseaseDefects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • DomainThe two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
  • Post-translational
    Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anemia, X-linked, without thrombocytopenia, included antibody
    • ERYF 1 antibody
    • Eryf1 antibody
    • Erythroid transcription factor antibody
    • Erythrold transcription factor 1 antibody
    • GATA 1 antibody
    • GATA binding factor 1 antibody
    • GATA binding protein 1 (globin transcription factor 1) antibody
    • GATA binding protein 1 antibody
    • GATA-1 antibody
    • GATA-binding factor 1 antibody
    • GATA1 antibody
    • GATA1_HUMAN antibody
    • GF 1 antibody
    • GF-1 antibody
    • GF1 antibody
    • Globin transcription factor 1 antibody
    • NF E1 antibody
    • NF E1 DNA binding protein antibody
    • NF-E1 DNA-binding protein antibody
    • NFE 1 antibody
    • NFE1 antibody
    • Nuclear factor erythroid 1 antibody
    • Transcription factor GATA1 antibody
    • XLANP antibody
    • XLTDA antibody
    • XLTT antibody
    see all

Anti-GATA1 (phospho S142) antibody images

  • ab28816 at (1:50-1:100), staining paraffin embedded human breast carcinoma. Left: Using GATA1 antibody (ab28816); Right: antibody preincubated with synthesized phosphopeptide.
  • Lane 1 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution
    Lane 2 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized non-phosphopeptide)
    Lane 3 : Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution (preincubated with synthesized phosphopeptide)

    Lane 1 : K562 cells
    Lane 2 : K562 cells
    Lane 3 : K562 cells

    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

References for Anti-GATA1 (phospho S142) antibody (ab28816)

ab28816 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. For this antibody, we saw a single band on Western blot when using k562 cell lysates. We did not test this antibody on bone marrow. 1) Did you use Tween-20 in the buffers? Tween-20 (0.05%) was added to the primary and...

Read More