Recombinant
RabMAb

Anti-GATA2 antibody [EPR2822(2)] (ab109241)

Overview

  • Product name
    Anti-GATA2 antibody [EPR2822(2)]
    See all GATA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2822(2)] to GATA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GATA2 aa 450-550. The exact sequence is proprietary.

  • Positive control
    • K562 cell lysate, Molt-4 cell lysate
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
    • Tissue specificity
      Endothelial cells.
    • Sequence similarities
      Contains 2 GATA-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DCML antibody
      • Endothelial transcription factor GATA 2 antibody
      • Endothelial transcription factor GATA-2 antibody
      • Endothelial transcription factor GATA2 antibody
      • FLJ45948 antibody
      • GATA 2 antibody
      • GATA binding protein 2 antibody
      • GATA-binding protein 2 antibody
      • Gata2 antibody
      • GATA2_HUMAN antibody
      • IMD21 antibody
      • MGC2306 antibody
      • MONOMAC antibody
      • NFE 1B antibody
      • NFE1B antibody
      • OTTHUMP00000216240 antibody
      see all

    Images

    • All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1/1000 dilution

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared using 1% SDS hot lysis method
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared using RIPA lysis method
      Lane 3 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 51 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST.

      The different result in K-562 is due to the lysates preparation method.

      For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

      The expression profile observed in U-937 is consistent with the literature (PMID: 19212333).
      Negative control: U-937 (PMID: 19212333)

    • All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Molt-4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 51 kDa

    References

    This product has been referenced in:
    • Bergiers I  et al. Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. Elife 7:N/A (2018). Read more (PubMed: 29555020) »
    • Fan J  et al. A novel role of CKIP-1 in promoting megakaryocytic differentiation. Oncotarget 8:30138-30150 (2017). Read more (PubMed: 28404913) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Thank you for your reply.

    I apologize for missing that.

    The homology with the immunogen sequence with mouse GATA3 is 78%.

    I hope this information helps. Please contact us with any other quesitons.

    For product ab109241 or ab3348-1, the sequence shows 100% homology with mouse, so we do predict it to cross-react.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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