Overview

  • Product name
  • Description
    Rabbit polyclonal to GATA3
  • Specificity
    At least two isoforms of GATA3 are known to exist; ab106625 will recognize both isoforms.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GATA3 (C terminal).
    Database link: P23771

  • Positive control
    • Human brain tissue lysate, Rat brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab106625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 - 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

Target

  • Function
    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificity
    T-cells and endothelial cells.
  • Involvement in disease
    Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similarities
    Contains 2 GATA-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Images

  • Lane 1 : Anti-GATA3 antibody (ab106625) at 1 µg/ml
    Lane 2 : Anti-GATA3 antibody (ab106625) at 2 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 48 kDa
  • ab106625, at 2.5 ug/ml, staining GATA3 in paraffin embedded Rat brain tissue by Immunohistochemistry.
  • Immunohistochemical analysis of rat brain tissue labelling GATA3 with ab106625 at 20 ug/ml.

References

This product has been referenced in:
  • de Souza AWS  et al. M2 macrophage is the predominant phenotype in airways inflammatory lesions in patients with granulomatosis with polyangiitis. Arthritis Res Ther 19:100 (2017). IHC-P ; Human . Read more (PubMed: 28521792) »
  • Zhong Y & Yi C MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3. Biosci Rep 36:N/A (2016). Read more (PubMed: 27354564) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Sample
Human Cell lysate - whole cell (Jurkat cell lysate)
Specification
Jurkat cell lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 24 2015

Thank you for contacting us. I have checked our catalog and found 4 anti-GATA3 antibodies that have been tested in mouse (ab32858, ab106625, ab82828 and ab110093), however none of these antibodies has been tested so far in frozen tissue section...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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