Overview

  • Product nameAnti-GATA3 antibody
    See all GATA3 primary antibodies
  • Description
    Goat polyclonal to GATA3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    EVTADQPRWVSHH

    conjugated to KLH, corresponding to N terminal amino acids 2-14 of Human GATA3 (CAL37566.1).

  • Positive control
    • Human Heart and Human Placenta tissues, MOLT-4 lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificityT-cells and endothelial cells.
  • Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Anti-GATA3 antibody images

  • Anti-GATA3 antibody (ab113519) at 3 µg/ml + MOLT-4 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 48 kDa
  • Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab113519 at a concentration of 3.75 µg/ml.
  • Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab113519 at a concentration of 3.75 µg/ml.

References for Anti-GATA3 antibody (ab113519)

ab113519 has not yet been referenced specifically in any publications.

Product Wall

I am sorry to see that the Abcam anti-GATA3 gave non-specific staining compared to the other antibody, but at least that antibody is working for you. I have issued a credit for the original purchase, with the assumption that you will need an Abcam anti...

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OK, I have left the order in place. I expect it will be delivered early next week.

We encourage you to send a review of your mouse and/or human results, positive or negative, via our Abreview program. Reviews earn credits towards future purc...

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We do not have any sample sizes of this or any of our other antibodies. If you have human xenografts that you were planning on testing anyway, those would serve as positive controls, and would allow me to issue a refund for the original order if ab1135...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"