Overview

  • Product name
  • Description
    Rabbit polyclonal to GATA3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human GATA3 (NP_002042.1).

  • Positive control
    • Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab124288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
    • Tissue specificity
      T-cells and endothelial cells.
    • Involvement in disease
      Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
    • Sequence similarities
      Contains 2 GATA-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Form
      There are 2 isoforms produced by alternative splicing.
    • Alternative names
      • GATA 3 antibody
      • GATA binding factor 3 antibody
      • GATA binding protein 3 antibody
      • GATA-binding factor 3 antibody
      • Gata3 antibody
      • GATA3_HUMAN antibody
      • HDR antibody
      • HDRS antibody
      • MGC2346 antibody
      • MGC5199 antibody
      • MGC5445 antibody
      • Trans acting T cell specific transcription factor GATA 3 antibody
      • Trans-acting T-cell-specific transcription factor GATA-3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling GATA3 with ab124288 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-GATA3 antibody (ab124288) at 0.4 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg

      Developed using the ECL technique

      Exposure time : 3 minutes

    References

    ab124288 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124288.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up