Overview

  • Product name
  • Description
    Rabbit polyclonal to GATA3
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (human) conjugated to KLH

  • Positive control
    • Recombinant GATA3 protein expressed in induced culture of E.coli (one shot Top10 competent cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab82828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
    • Tissue specificity
      T-cells and endothelial cells.
    • Involvement in disease
      Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
    • Sequence similarities
      Contains 2 GATA-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Form
      There are 2 isoforms produced by alternative splicing.
    • Alternative names
      • GATA 3 antibody
      • GATA binding factor 3 antibody
      • GATA binding protein 3 antibody
      • GATA-binding factor 3 antibody
      • Gata3 antibody
      • GATA3_HUMAN antibody
      • HDR antibody
      • HDRS antibody
      • MGC2346 antibody
      • MGC5199 antibody
      • MGC5445 antibody
      • Trans acting T cell specific transcription factor GATA 3 antibody
      • Trans-acting T-cell-specific transcription factor GATA-3 antibody
      see all

    Images

    • All lanes : Anti-GATA3 antibody (ab82828) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)

    References

    ab82828 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us. I have checked our catalog and found 4 anti-GATA3 antibodies that have been tested in mouse (ab32858, ab106625, ab82828 and ab110093), however none of these antibodies has been tested so far in frozen tissue section...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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