Overview

  • Product nameAnti-GATA3 antibody
    See all GATA3 primary antibodies
  • Description
    Rabbit polyclonal to GATA3
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (MDAAQYPLPE EVDVLFNIDG QGNHVPPYYG NSVRATVQRY PPTHHGSQVC) of human GATA3 (NP_002042).

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 mg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-GATA3 antibody images

  • Anti-GATA3 antibody (ab83319) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Gel Concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GATA3 with ab83319 at 5.0µg/ml.

  • All lanes : Anti-GATA3 antibody (ab83319) at 1 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate with blocking peptide at 5 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 48 kDa

References for Anti-GATA3 antibody (ab83319)

ab83319 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"