Overview

  • Product nameAnti-GATA3 antibody
    See all GATA3 primary antibodies
  • Description
    Mouse monoclonal to GATA3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: LGSHHTASPW NLSPFSKTSI HHGSPGPLSV YPPASSSSLS GGHASPHLFT FPPTPPKDVS PDPSLSTPGS AGSARQDEKE CLKYQVPLPD SMKLESSH, corresponding to amino acids 103-200 of human GATA3 (NP_001002295), with a proprietary tag

  • Positive control
    • The immunogen; HeLa nuclear extract; Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificityT-cells and endothelial cells.
  • Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Anti-GATA3 antibody images

  • Anti-GATA3 antibody (ab89776) at 1 µg/ml + immunogen at 0.2 µg

    Predicted band size : 48 kDa
  • Anti-GATA3 antibody (ab89776) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Anti-GATA3 antibody (ab89776) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
  • Detection limit for ab89776 is approximately 0.03 ng/ml as a capture antibody.

References for Anti-GATA3 antibody (ab89776)

ab89776 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"