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Mouse monoclonal [mAbcam77073] to GATA3
Predicted to work with:
Mouse, Rat, Chicken, Cow, Pig
Synthetic peptide derived from within residues 1 - 100 of Human GATA3.
(Peptide available as
This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.
This antibody clone is manufactured by Abcam.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
T-cells and endothelial cells.
Involvement in disease
Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Contains 2 GATA-type zinc fingers.
Information by UniProt
There are 2 isoforms produced by alternative splicing.
GATA 3 antibody
GATA binding factor 3 antibody
GATA binding protein 3 antibody
Western blot - GATA3 antibody [mAbcam77073] (ab77073)
All lanes :
Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml
Lane 1 :
Human thymus tissue lysate - total protein (
Lane 2 :
Human lymph node tissue lysate - total protein (
Lane 3 :
Human spleen tissue lysate - total protein (
Lane 4 :
Tonsil (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
55 kDa (
why is the actual band size different from the predicted?
Additional bands at :
90 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"