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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Purity IgG fraction
Clone number mAbcam77073
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificity T-cells and endothelial cells.
Involvement in disease Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Sequence similarities Contains 2 GATA-type zinc fingers.
Cellular localization Nucleus.
Information by UniProt
Form There are 2 isoforms produced by alternative splicing.
GATA 3 antibody
GATA binding factor 3 antibody
GATA binding protein 3 antibody
Anti-GATA3 antibody [mAbcam77073] images
Western blot - GATA3 antibody [mAbcam77073] (ab77073)
All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml Lane 1 : Human thymus tissue lysate - total protein ( ab30146) Lane 2 : Human lymph node tissue lysate - total protein ( ab29871) Lane 3 : Human spleen tissue lysate - total protein ( ab29699) Lane 4 : Tonsil (Human) Tissue Lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed ( ab97040) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 48 kDa Observed band size : 55 kDa ( why is the actual band size different from the predicted?) Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands. Exposure time : 12 minutes
References for Anti-GATA3 antibody [mAbcam77073] (ab77073)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"