Anti-GATA3 antibody [mAbcam77073] (ab77073)

Overview

  • Product nameAnti-GATA3 antibody [mAbcam77073]
    See all GATA3 primary antibodies
  • Description
    Mouse monoclonal [mAbcam77073] to GATA3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human GATA3.

    (Peptide available as ab107920.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab77073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

Target

Anti-GATA3 antibody [mAbcam77073] images

  • All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Tonsil (Human) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-GATA3 antibody [mAbcam77073] (ab77073)

ab77073 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"