Anti-GATA3 (phospho S308) antibody (ab61052)


  • Product nameAnti-GATA3 (phospho S308) antibody
    See all GATA3 primary antibodies
  • Description
    Rabbit polyclonal to GATA3 (phospho S308)
  • SpecificityDdetects endogenous levels of GATA3 only when phosphorylated at serine 308.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human GATA3 around the phosphorylation site of serine 308 (R-L-SP-A-A)

  • Positive control
    • HuvEc cells



Our Abpromise guarantee covers the use of ab61052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 21930963
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.


  • FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificityT-cells and endothelial cells.
  • Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Anti-GATA3 (phospho S308) antibody images

  • 1/500-1/1000 ab61052 staining phosphorilated GATA3 on HuvEc cells, in the absence (left) or presence (right) of the immunizing phosphopeptide.

References for Anti-GATA3 (phospho S308) antibody (ab61052)

This product has been referenced in:
  • Schiering C  et al. The alarmin IL-33 promotes regulatory T-cell function in the intestine. Nature N/A:N/A (2014). Read more (PubMed: 25043027) »
  • Izzo F  et al. Progesterone receptor activation downregulates GATA3 by transcriptional repression and increased protein turnover promoting breast tumor growth. Breast Cancer Res 16:491 (2014). WB ; Human . Read more (PubMed: 25479686) »

See all 4 Publications for this product

Product Wall

Thanks for contacting us and for your interest in these products. We do accept AMEX credit cards as payment. I created a proforma for you which you should receive in a separate email, but we will need a full billing address in order to process t...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (T47D human breast cancer cell line)
Permeabilization Yes - permeabilization buffer (PB) (PBS 10% FCS 0.5% saponine)
Gating Strategy No gating was performed. All the cells positive with propidium iodide staining were selected.
Specification T47D human breast cancer cell line
Fixation Paraformaldehyde

Dr. Cecilia PROIETTI

Verified customer

Submitted Jan 27 2016

DISCOUNT CODE: *** Expiration date: January 16, 2012 I am very pleased to hear you would like to accept our offer and test ab61052 in Western blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer...

Read More
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse stromal cell line)
Loading amount 40 µg
Specification mouse stromal cell line
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 18 2011