Anti-GATA3 (phospho S308) antibody (ab61052)

Overview

  • Product nameAnti-GATA3 (phospho S308) antibody
    See all GATA3 primary antibodies
  • Description
    Rabbit polyclonal to GATA3 (phospho S308)
  • SpecificityDdetects endogenous levels of GATA3 only when phosphorylated at serine 308.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human GATA3 around the phosphorylation site of serine 308 (R-L-SP-A-A)

  • Positive control
    • HuvEc cells

Properties

Applications

Our Abpromise guarantee covers the use of ab61052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 21930963
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificityT-cells and endothelial cells.
  • Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Anti-GATA3 (phospho S308) antibody images

  • 1/500-1/1000 ab61052 staining phosphorilated GATA3 on HuvEc cells, in the absence (left) or presence (right) of the immunizing phosphopeptide.

References for Anti-GATA3 (phospho S308) antibody (ab61052)

This product has been referenced in:
  • Schiering C  et al. The alarmin IL-33 promotes regulatory T-cell function in the intestine. Nature N/A:N/A (2014). Read more (PubMed: 25043027) »
  • Izzo F  et al. Progesterone receptor activation downregulates GATA3 by transcriptional repression and increased protein turnover promoting breast tumor growth. Breast Cancer Res 16:491 (2014). WB ; Human . Read more (PubMed: 25479686) »

See all 4 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (T47D human breast cancer cell line)
Permeabilization Yes - permeabilization buffer (PB) (PBS 10% FCS 0.5% saponine)
Gating Strategy No gating was performed. All the cells positive with propidium iodide staining were selected.
Specification T47D human breast cancer cell line
Fixation Paraformaldehyde
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Dr. Cecilia PROIETTI

Verified customer

Submitted Jan 27 2016

DISCOUNT CODE: *** Expiration date: January 16, 2012 I am very pleased to hear you would like to accept our offer and test ab61052 in Western blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer...

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Application Western blot
Sample Mouse Cell lysate - whole cell (mouse stromal cell line)
Loading amount 40 µg
Specification mouse stromal cell line
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted May 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"