Anti-GATA3 (phospho S308) antibody (ab61052)

Overview

  • Product name
    Anti-GATA3 (phospho S308) antibody
    See all GATA3 primary antibodies
  • Description
    Rabbit polyclonal to GATA3 (phospho S308)
  • Specificity
    Ddetects endogenous levels of GATA3 only when phosphorylated at serine 308.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human GATA3 around the phosphorylation site of serine 308 (R-L-SP-A-A)

  • Positive control
    • HuvEc cells

Properties

Applications

Our Abpromise guarantee covers the use of ab61052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 21930963
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • Function
    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificity
    T-cells and endothelial cells.
  • Involvement in disease
    Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similarities
    Contains 2 GATA-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Images

  • 1/500-1/1000 ab61052 staining phosphorilated GATA3 on HuvEc cells, in the absence (left) or presence (right) of the immunizing phosphopeptide.

References

This product has been referenced in:

See all 6 Publications for this product

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (T47D human breast cancer cell line)
Permeabilization
Yes - permeabilization buffer (PB) (PBS 10% FCS 0.5% saponine)
Gating Strategy
No gating was performed. All the cells positive with propidium iodide staining were selected.
Specification
T47D human breast cancer cell line
Fixation
Paraformaldehyde
Username

Dr. Cecilia Proietti

Verified customer

Submitted Jan 27 2016

DISCOUNT CODE: *** Expiration date: January 16, 2012 I am very pleased to hear you would like to accept our offer and test ab61052 in Western blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer...

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse stromal cell line)
Loading amount
40 µg
Specification
mouse stromal cell line
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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