Overview

  • Product name
  • Description
    Rabbit polyclonal to GATA4
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment within Human GATA4 (internal sequence). The exact sequence is proprietary.
    Database link: P43694

  • Positive control
    • WB: HeLa whole cell extract; NIH/3T3, JC, BCL-1, HeLa and A549 whole cell lysates; Rat heart lysate. ICC/IF: HeLa cells. IHC-P: Mouse lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • Involvement in disease
    Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities
    Contains 2 GATA-type zinc fingers.
  • Post-translational
    modifications
    Methylation at Lys-300 attenuates transcriptional activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Images

  • All lanes : Anti-GATA4 antibody (ab227512) at 1/3000 dilution

    Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : GATA4 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 45 kDa

    10% SDS-PAGE gel.

  • Paraffin-embedded mouse lung tissue stained for GATA4 with ab227512 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GATA4 (green) using ab227512 at 1/500 dilution in ICC/IF.

    Counterstain: Hoechst 33342 (blue).

  • Anti-GATA4 antibody (ab227512) at 1/1000 dilution + Rat heart lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 45 kDa

    10% SDS-PAGE gel.

  • All lanes : Anti-GATA4 antibody (ab227512) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 45 kDa

    10% SDS PAGE gel.

  • All lanes : Anti-GATA4 antibody (ab227512) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 45 kDa

    10% SDS-PAGE gel.

References

ab227512 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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