Overview

  • Product nameAnti-GATA4 antibody
    See all GATA4 primary antibodies
  • Description
    Rabbit polyclonal to GATA4
  • SpecificityDetects total GATA4 levels.
  • Tested applicationsSuitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human GATA4 around the phosphorylation site of serine 262 (R-L-SP-A-S).

  • Positive control
    • HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • Involvement in diseaseAtrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Post-translational
    modifications
    Methylation at Lys-300 attenuates transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 antibody images

  • 1/500-1/1000 ab61170 staining GATA4 on HeLa cells, in the absence or presence of the immunizing peptide.

References for Anti-GATA4 antibody (ab61170)

This product has been referenced in:
  • Oltolina F  et al. Human Cardiac Progenitor Spheroids Exhibit Enhanced Engraftment Potential. PLoS One 10:e0137999 (2015). ICC/IF ; Human . Read more (PubMed: 26375957) »
  • Huang WC  et al. Treatment of Glucocorticoids Inhibited Early Immune Responses and Impaired Cardiac Repair in Adult Zebrafish. PLoS One 8:e66613 (2013). ICC/IF . Read more (PubMed: 23805247) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (differentiated iPSC)
Specification differentiated iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Apr 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"