Overview

  • Product nameAnti-GATA4 antibody
    See all GATA4 primary antibodies
  • Description
    Rabbit polyclonal to GATA4
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Rhesus monkey
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human GATA4.

    (Peptide available as ab94555.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab84593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/50.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • Involvement in diseaseAtrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Post-translational
    modifications
    Methylation at Lys-300 attenuates transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 antibody images

  • All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ab84593 staining GATA4 (green) in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized in 1% Triton X-100 and blocked with 10% goat serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 10% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Red - Myh6/7, Blue - DAPI.

    See Abreview

  • Immunofluorescence image of Gata4 (ab84593) on human embryonic stem cell derived cardiomyocytes. The cells were fixed in methanol and permeabilized in 0.1% Triton-X-100. In this image you can see a cardiomyocyte stained in red with a cardiac alpha actinin antibody and both the cardiomyocyte and a fibroblast stained in green with ab84593.

    See Abreview

  • All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human Fetal Heart - Total Protein Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GATA4 antibody (ab84593)

This product has been referenced in:
  • Wang H  et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
  • Kanatsu-Shinohara M  et al. Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc Natl Acad Sci U S A 111:8826-31 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24879440) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Specification heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Formaldehyde
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Submitted Feb 13 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification Cardiomyocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Feb 11 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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Submitted Sep 19 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Sample Mouse Tissue sections (mouse embryo)
Specification mouse embryo
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde
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Submitted Jan 03 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrition buffer
Sample Mouse Tissue sections (mouse embryo)
Specification mouse embryo
Permeabilization Yes - 1% triton x-100
Fixative Formaldehyde
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Submitted Jan 02 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12)
Sample Mouse Cell lysate - nuclear (Gata4 over-expressing undifferentiated mouse ES ce)
Specification Gata4 over-expressing undifferentiated mouse ES ce
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Ms. Elisa Ponsele

Verified customer

Submitted Dec 20 2013

Thank you for contacting us. Your credit note ID is xxxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice...

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Thank you for your reply. You do not need to return the antibodies which you have already received. Further we are very happy to send you new products against the same target or a different target. You do mentionAnti Neurofibromin ab17963, and I wou...

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Thank you for your reply.

Based on the information that you have provided, I do not think there is anymore protocol advice that I could supply that would help resolve the issue you are having with these two antibodies in western blot. Therefor...

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Thank you for your reply and for providing that information.

For ab84593, the band that you are seeing at just above 50kDa, is very intense and I am wondering if that could be the correct band, but is just running a little higher than expected...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"