Recombinant
RabMAb

Anti-GATA4 antibody [EPR4768] (ab134057)

Overview

  • Product name
    Anti-GATA4 antibody [EPR4768]
    See all GATA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR4768] to GATA4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GATA4 aa 1-100 (N terminal).
    Database link: P43694

  • Positive control
    • HepG2, Caco-2 and HeLa cell lysates; HepG2 cells.
  • General notes

    This antibody was developed as part of a collaboration between Dartmouth College and the lab of Sergei Tevosian.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
Flow Cyt 1/600.

For unpurified use at 1/1200.

 

 

 

 

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/150.

For unpurified use at 1/100 - 1/250.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
    • Involvement in disease
      Atrial septal defect 2
      Ventricular septal defect 1
      Tetralogy of Fallot
      Atrioventricular septal defect 4
      Testicular anomalies with or without congenital heart disease
      GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities
      Contains 2 GATA-type zinc fingers.
    • Post-translational
      modifications
      Methylation at Lys-300 attenuates transcriptional activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ASD2 antibody
      • GATA 4 antibody
      • GATA binding protein 4 antibody
      • GATA-binding factor 4 antibody
      • gata4 antibody
      • GATA4_HUMAN antibody
      • MGC126629 antibody
      • Transcription factor GATA 4 antibody
      • Transcription factor GATA-4 antibody
      • Transcription factor GATA4 antibody
      • VSD1 antibody
      see all

    Images

    • Overlay histogram showing Caco-2 cells stained with ab134057 (red line) at 1/600 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

    • ab134057 staining GATA4 in the PC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).  An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    • Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + Mouse spleen lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 54 kDa

    • Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + HepG2 lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated

      Predicted band size: 54 kDa

    • Overlay histogram showing HeLa cells stained with ab134057, unpurified (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134057, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • All lanes : Anti-GATA4 antibody [EPR4768] (ab134057) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : Caco-2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 54 kDa

    • Immunofluorescent analysis of HepG2 cells labelling GATA4 with ab134057, unpuified, at 1/100 dilution.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Karimzadeh F & Opas M Calreticulin Is Required for TGF-ß-Induced Epithelial-to-Mesenchymal Transition during Cardiogenesis in Mouse Embryonic Stem Cells. Stem Cell Reports 8:1299-1311 (2017). WB ; Mouse . Read more (PubMed: 28434939) »
    • Mundre RS  et al. Synergistic role of 5-azacytidine and ascorbic acid in directing cardiosphere derived cells to cardiomyocytes in vitro by downregulating Wnt signaling pathway via phosphorylation of ß-catenin. PLoS One 12:e0188805 (2017). Read more (PubMed: 29190771) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Specification
    Human pluripotent stem cell derived cardiomyocyte
    Permeabilization
    Yes - saponin
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 19 2014

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (10% bottom gel, 4% top gel)
    Sample
    Sheep Tissue lysate - whole (cardiac muscle - left ventricle)
    Specification
    cardiac muscle - left ventricle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Adrienne Wilburn

    Verified customer

    Submitted Jul 14 2014

    Thanks your for your email.
    I am sorry to hear that the results does not improve. These antibodies has been positively tested so we are unsure about the cause of no bands.
    I can provide you replacement antibodies if you are interested in using...

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    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
    I have read the details you have kindly provided and have following further suggestions for improvements;
    It is always good to try the ant...

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    Thank you for contacting us.
    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    I am attaching ...

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    Thank you for contacting us.

    ab134057 and ab86371 have not been purified and therefore, their concentration has not been determined.

    However we predict that ab134057, which is a tissue culture supernatant, will have a concentration...

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