Anti-GATA4 (phospho S105) antibody (ab5245)

Overview

  • Product nameAnti-GATA4 (phospho S105) antibody
    See all GATA4 primary antibodies
  • Description
    Rabbit polyclonal to GATA4 (phospho S105)
  • Tested applicationsSuitable for: ICC/IF, EMSA, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog
    Predicted to work with: Chicken, Human
  • Immunogen

    Synthetic peptide (Human) derived from a region of GATA 4 that contains serine 105.

  • Positive control
    • Phenylephrine (PE)-stimulated rat cardiomyocytes, transfected with mouse GATA 4.

Properties

Applications

Our Abpromise guarantee covers the use of ab5245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19546173
EMSA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22919071
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).

Target

  • FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • Involvement in diseaseAtrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Post-translational
    modifications
    Methylation at Lys-300 attenuates transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 (phospho S105) antibody images



  • Predicted band size : 46 kDa


    Extracts prepared from cardiomyocytes overexpressing wild-type GATA-4 (1-4 from left to right) stimulated with phenylephrine (PE) for 24 hours were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.35 µg/mL ab5245 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to GATA-4 [pS105] blocks the antibody signal, and that the S105A mutant does not react, thereby demonstrating the specificity of the antibody.

References for Anti-GATA4 (phospho S105) antibody (ab5245)

This product has been referenced in:
  • Tasoglu S  et al. Guided and magnetic self-assembly of tunable magnetoceptive gels. Nat Commun 5:4702 (2014). ICC/IF ; Rat . Read more (PubMed: 25175148) »
  • Bisping E  et al. Transcription Factor GATA4 Is Activated but Not Required for Insulin-like Growth Factor 1 (IGF1)-induced Cardiac Hypertrophy. J Biol Chem 287:9827-34 (2012). WB ; Rat . Read more (PubMed: 22228770) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (pancreatic cancer)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification pancreatic cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Feb 09 2016

Thank you for your protocol details, I'm very sorry to hear you are experiencing problems with ab5245 and would like to recommend the following advice: 1) please make sure that the protein is in its phosphorylated form: do you have a stimulation pa...

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