Overview

  • Product nameAnti-GATA4 (phospho S105) antibody
    See all GATA4 primary antibodies
  • Description
    Rabbit polyclonal to GATA4 (phospho S105)
  • SpecificityThis antibody detects endogenous levels of GATA4 only when phosphorylated at serine 105.
  • Tested applicationsSuitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthesized phosphopeptide derived from human GATA4 around the phosphorylation site of serine 105 (PVSPPR).

  • Positive control
    • HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.

Target

  • FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • Involvement in diseaseAtrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Post-translational
    modifications
    Methylation at Lys-300 attenuates transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 (phospho S105) antibody images

  • Immunofluorescence analysis of HepG2 cells using ab63398 at a 1:500.
    Left image untreated.
    Right image treated with phospho peptide.

References for Anti-GATA4 (phospho S105) antibody (ab63398)

This product has been referenced in:
  • Schunke KJ  et al. Acetaminophen attenuates doxorubicin-induced cardiac fibrosis via osteopontin and GATA4 regulation: reduction of oxidant levels. J Cell Physiol 228:2006-14 (2013). IHC-P ; Mouse . Read more (PubMed: 23526585) »
  • Shinozawa T  et al. A novel purification method of murine embryonic stem cell- and human-induced pluripotent stem cell-derived cardiomyocytes by simple manual dissociation. J Biomol Screen 17:683-91 (2012). ICC/IF ; Mouse . Read more (PubMed: 22274911) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"