Anti-GATA4 (phospho S262) antibody (ab61053)

Overview

  • Product nameAnti-GATA4 (phospho S262) antibody
    See all GATA4 primary antibodies
  • Description
    Rabbit polyclonal to GATA4 (phospho S262)
  • SpecificityDdetects endogenous levels of GATA4 only when phosphorylated at serine 262.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human GATA4 around the phosphorylation site of serine 262 (R-L-SP-A-S)

  • Positive control
    • HuvEc cells

Properties

Applications

Our Abpromise guarantee covers the use of ab61053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • Involvement in diseaseAtrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesContains 2 GATA-type zinc fingers.
  • Post-translational
    modifications
    Methylation at Lys-300 attenuates transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 (phospho S262) antibody images



  • Predicted band size : 44 kDa

    Western blot analysis of lysates from 293 cells, using GATA4 (Phospho-Ser262) Antibody (ab61053). The lane on the right is blocked with the phospho peptide.

  • 1/500-1/1000 ab61053 staining phosphorilated GATA4 on HuvEc cells in the absence (left) or presence (right) of the immunizing phosphopeptide.

References for Anti-GATA4 (phospho S262) antibody (ab61053)

ab61053 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"