Overview

  • Product name
  • Description
    Rabbit polyclonal to GATA5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: SGCEPSPQPP ELAARPGWAQ TATADSSAFG PGSPHPPAAH PPGATAFPFA, corresponding to N terminal amino acids 47-96 of Human GATA5

  • Positive control
    • HepG2 cell lysate; Human skeletal muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-GATA5 antibody images

  • Anti-GATA5 antibody (ab118777) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 25 kDa
  • Immunohistochemistry (Formalin-PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling GATA5 with ab118777 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

References for Anti-GATA5 antibody (ab118777)

ab118777 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab118777.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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