Overview

  • Product nameAnti-GATA5 antibody
    See all GATA5 primary antibodies
  • Description
    Rabbit polyclonal to GATA5
  • SpecificityThis antibody recognizes GATA5
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    A region within synthetic peptide: LNSESSATTL KAESSLASPV CAGPTITSQA SSPADESLAS SHLEFKFEPE, corresponding to C terminal amino acids 325-374 of Human GATA5

  • Positive control
    • SP2/0 lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF 1/100.

Target

Anti-GATA5 antibody images

  • Anti-GATA5 antibody (ab48820) at 1.25 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • ab48820 staining GATA5 in murine epicardial explant culture by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using 0.5% Triton X-100 in PBS, blocked with 1% goat serum 10% BSA 0.1% Triton X-100 PBS and then incubated with ab48820 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit IgG (H+L) used at a 1/200 dilution.

    See Abreview

References for Anti-GATA5 antibody (ab48820)

This product has been referenced in:
  • Risebro CA  et al. Characterisation of the human embryonic and foetal epicardium during heart development. Development 142:3630-6 (2015). IHC ; Human . Read more (PubMed: 26395486) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (epicardial explant culture)
Specification epicardial explant culture
Fixative Formaldehyde
Permeabilization Yes - 0.5% Tx100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Jun 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"