Anti-Gata6 antibody (ab22600)
Key features and details
- Rabbit polyclonal to Gata6
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Gata6 antibody
See all Gata6 primary antibodies -
Description
Rabbit polyclonal to Gata6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Chicken, Cow, Human, Pig -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent. |
Target
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Function
Thought to be important for regulating terminal differentiation and/or proliferation. -
Tissue specificity
Expressed in heart, gut and gut-derived tissues. -
Sequence similarities
Contains 2 GATA-type zinc fingers. -
Domain
The GATA-type zinc fingers mediate interaction with LMCD1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2627 Human
- Entrez Gene: 14465 Mouse
- Entrez Gene: 397600 Pig
- Entrez Gene: 29300 Rat
- Omim: 601656 Human
- SwissProt: P43693 Chicken
- SwissProt: Q92908 Human
- SwissProt: Q61169 Mouse
see all -
Alternative names
- Gata binding factor 6 antibody
- Gata binding protein 6 antibody
- GATA-binding factor 6 antibody
see all
Images
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Anti-Gata6 antibody (ab22600) at 1 µg/ml + D3 ES Cells Day 15 EB (Gata6 +ve) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab22600 has been referenced in 28 publications.
- Israeli M et al. Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2. Nat Commun 13:2237 (2022). PubMed: 35469023
- Pérez-Gómez A et al. Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates. Front Vet Sci 8:680539 (2021). PubMed: 34212020
- Wang C et al. Loss of GATA6 expression promotes lymphatic metastasis in bladder cancer. FASEB J 34:5754-5766 (2020). PubMed: 32103545
- Cao Y et al. HYDIN loss-of-function inhibits GATA4 expression and enhances atrial septal defect risk. Mech Dev 162:103611 (2020). PubMed: 32376282
- Li Y et al. Derivation of porcine extraembryonic endoderm-like cells from blastocysts. Cell Prolif 53:e12782 (2020). PubMed: 32196806