Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab39123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

Anti-Gata6 antibody images

  • Anti-Gata6 antibody (ab39123) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 30 kDa,60 kDa (possible isoform),70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Gata6 antibody (ab39123)

This product has been referenced in:
  • Kee HJ  et al. HDAC inhibition suppresses cardiac hypertrophy and fibrosis in DOCA-salt hypertensive rats via regulation of HDAC6/HDAC8 enzyme activity. Kidney Blood Press Res 37:229-39 (2013). Rat . Read more (PubMed: 23868068) »
  • Barbuti A  et al. Mesoangioblasts from ventricular vessels can differentiate in vitro into cardiac myocytes with sinoatrial-like properties. J Mol Cell Cardiol 48:415-23 (2010). Read more (PubMed: 19837079) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (differentiated mouse ES cell (D3 line) day 14)
Specification
differentiated mouse ES cell (D3 line) day 14
Preparation
Cell harvesting/tissue preparation method: trypsinisation of EBs to single cell suspension
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
Yes - 70% methanol
Gating Strategy
FSC vs SSC on negative control sample
Username

Dr. Lisa Mohamet

Verified customer

Submitted Jun 04 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (differentiated mouse EBs (day14))
Loading amount
20 µg
Specification
differentiated mouse EBs (day14)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Differentiated embryonic stem cells, feeder free)
Specification
Differentiated embryonic stem cells, feeder free
Fixative
Paraformaldehyde
Permeabilization
Yes - see blocking buffer below
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Differentiated mouse ES cell (D3 line))
Specification
Differentiated mouse ES cell (D3 line)
Fixative
Paraformaldehyde
Permeabilization
Yes - see blocking buffer below
Blocking step
0.1%BSA, 1%serum, 0.1% triton in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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