Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab39123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human IRF5 peptide (ab22411) or Human Gata6 peptide (ab24411).

Target

Anti-Gata6 antibody images

  • Anti-Gata6 antibody (ab39123) at 1 µg/ml + Heart (Human) Tissue Lysate - adult normal tissue (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 30 kDa,60 kDa (possible isoform),70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Gata6 antibody (ab39123)

This product has been referenced in:
  • Barbuti A  et al. Mesoangioblasts from ventricular vessels can differentiate in vitro into cardiac myocytes with sinoatrial-like properties. J Mol Cell Cardiol 48:415-23 (2010). Read more (PubMed: 19837079) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Sample Mouse Cell (differentiated mouse ES cell (D3 line) day 14)
Specification differentiated mouse ES cell (D3 line) day 14
Preparation Cell harvesting/tissue preparation method: trypsinisation of EBs to single cell suspension
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation Paraformaldehyde
Permeabilization Yes - 70% methanol
Gating Strategy FSC vs SSC on negative control sample
Username

Dr. lisa mohamet

Verified customer

Submitted Jun 04 2010

Application Western blot
Sample Mouse Cell lysate - other (differentiated mouse EBs (day14))
Loading amount 20 µg
Specification differentiated mouse EBs (day14)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Differentiated embryonic stem cells, feeder free)
Specification Differentiated embryonic stem cells, feeder free
Fixative Paraformaldehyde
Permeabilization Yes - see blocking buffer below
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Differentiated mouse ES cell (D3 line))
Specification Differentiated mouse ES cell (D3 line)
Fixative Paraformaldehyde
Permeabilization Yes - see blocking buffer below
Blocking step 0.1%BSA, 1%serum, 0.1% triton in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"