Overview

  • Product nameAnti-Gata6 antibody
    See all Gata6 primary antibodies
  • Description
    Rabbit polyclonal to Gata6
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide within residues: GAGAPVMTGA GESTNPENSE LKYSGQDGLY IGVSLASPAE VTSSVRPDSW, corresponding to internal sequence amino acids 540-589 of Human Gata6 (NP_005248)

  • Positive control
    • MCF7 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

Target

Anti-Gata6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Gata6 with ab86271 at 5ug/ml.

  • Anti-Gata6 antibody (ab86271) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,41 kDa. We are unsure as to the identity of these extra bands.
  • ab86271 staining Gata6 in human embryonic stem cells (H1 differentiated to HN4 positive hepatic progenitor cells) by Immunocytochemistry/ Immunofluorescence. Right image HNF4.

References for Anti-Gata6 antibody (ab86271)

ab86271 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"