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Synthetic peptide within residues: GAGAPVMTGA GESTNPENSE LKYSGQDGLY IGVSLASPAE VTSSVRPDSW, corresponding to internal sequence amino acids 540-589 of Human Gata6 (NP_005248)
Our Abpromise guarantee covers the use of ab86271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Gata6 with ab86271 at 5ug/ml.
ab86271 has not yet been referenced specifically in any publications.