Overview

  • Product nameAnti-GBA2 antibody
    See all GBA2 primary antibodies
  • Description
    Mouse polyclonal to GBA2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    GBA2 (NP_065995.1, 1 a.a. ~ 927 a.a) full-length human protein.

  • Positive control
    • GBA2 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

Target

  • RelevanceGBA2 is a non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide. It is involved in sphingomyelin generation and prevention of glycolipid accumulation. GBA2 may also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo.
  • Cellular localizationCell membrane; Single-pass membrane protein. Note=Not localized to lipid rafts.
  • Database links
  • Alternative names
    • AD035 antibody
    • Beta glucocerebrosidase 2 antibody
    • Beta glucosidase 2 antibody
    • DKFZp762K054 antibody
    • glucosidase, beta (bile acid) 2 antibody
    • Glucosylceramidase 2 antibody
    • KIAA1605 antibody
    • MGC16895 antibody
    • NLGase antibody
    • Non lysosomal glucosylceramidase antibody
    • SPG46 antibody
    see all

Anti-GBA2 antibody images

  • All lanes : Anti-GBA2 antibody (ab69366) at 1/500 dilution

    Lane 1 : GBA2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 105 kDa
    Observed band size : 105 kDa

References for Anti-GBA2 antibody (ab69366)

ab69366 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neuroblastoma cell line Neuro2a)
Permeabilization Yes - 0.1% PBS-Tween
Specification Neuroblastoma cell line Neuro2a
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Massimo Aureli

Verified customer

Submitted Apr 29 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Bronchial epithelial cells overexpressing GBA2)
Permeabilization Yes - 0.1% PBS-Tween
Specification Bronchial epithelial cells overexpressing GBA2
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Massimo Aureli

Verified customer

Submitted Apr 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"