Recombinant
RabMAb

Anti-GBGT1 antibody [EPR10467] (ab154837)

Overview

  • Product name
    Anti-GBGT1 antibody [EPR10467]
    See all GBGT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10467] to GBGT1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GBGT1 (Uniprot: Q8N5D6)

  • Positive control
    • Human placenta, ovary cancer, fetal heart and fetal lung lysates; HeLa and Jurkat cell lysates; Human kidney and pancreas tissues; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab154837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Catalyzes the formation of some glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to some substrate. Glycolipids probably serve for adherence of some pathogens.
    • Tissue specificity
      Widely expressed. Expressed at higher level in placenta, ovary and peripheral blood leukocyte, whereas it is weakly expressed in liver, thymus, and testis.
    • Pathway
      Protein modification; protein glycosylation.
    • Sequence similarities
      Belongs to the glycosyltransferase 6 family.
    • Domain
      The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
    • Cellular localization
      Golgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3-N-acetylgalactosaminyltransferase 1 antibody
      • A3GALNT antibody
      • EC=2.4.1.- antibody
      • Forssman glycolipid synthase-like protein antibody
      • Forssman glycolipid synthetase (FS) antibody
      • Forssman synthetase antibody
      • FS antibody
      • GBGT1 antibody
      • GBGT1_HUMAN antibody
      • Globoside alpha-1 antibody
      • globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 antibody
      • glycolipid synthase-like protein antibody
      • RP11-326L24.6 antibody
      • UNQ2513 antibody
      • UNQ2513/PRO6002 antibody
      see all

    Images

    • All lanes : Anti-GBGT1 antibody [EPR10467] (ab154837) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : Human fetal heart lysate
      Lane 4 : Human fetal lung lysate
      Lane 5 : HeLa cell lysate
      Lane 6 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling GBGT1 using ab154837 at a 1/100 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GBGT1 using ab154837 at a 1/100 dilution.
    • Flow cytometry analysis of permeabilized Jurkat cells labeling GBGT1 (red), using ab154837 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    ab154837 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154837.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up