Recombinant Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13206] to GBP2 - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GBP2 antibody [EPR13206] - N-terminal
See all GBP2 primary antibodies -
Description
Rabbit monoclonal [EPR13206] to GBP2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human GBP2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P32456 -
Positive control
- WB: Human spleen, IM-9, HACAT, A549 and K562 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13206 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 67 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 67 kDa. |
Target
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Relevance
Guanylate-binding proteins (GBPs) are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP. -
Cellular localization
Cell membrane; Lipid-anchor; Cytoplasmic side -
Database links
- Entrez Gene: 2634 Human
- Omim: 600412 Human
- SwissProt: P32456 Human
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Alternative names
- GBP 2 antibody
- GTP binding protein 2 antibody
- Guanine nucleotide binding protein 2 antibody
see all
Images
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All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : GBP2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HaCaT (Human keratinocyte cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaLanes 1-4: Merged signal (red and green). Green - ab179829 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.
ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267218 (knockout cell lysate ab257962) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : GBP2 knockout A549 cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : HaCaT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab179829 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.
ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267219 (knockout cell lysate ab257963) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution
Lane 1 : Human spleen cell lysate
Lane 2 : IM-9 cell lysate
Lane 3 : HACAT cell lysate
Lane 4 : K562 cell lysate
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 67 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab179829 has been referenced in 1 publication.
- Ren Y et al. GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling. Cell Death Discov 8:208 (2022). PubMed: 35436989