Overview

  • Product name
    GBR 12909 dihydrochloride
  • Description
    Selective dopamine transport inhibitor
  • Biological description
    Potent, selective dopamine reuptake inhibitor (IC50 = 2.32 µM in rat hippocampal slices; IC50 = 6-35 µM in whole cell patch clamp recordings).
  • Purity
    > 98%

Properties

Applications

Our Abpromise guarantee covers the use of ab120607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab65783 staining NR2B in SKNSH cells treated with GBR 12909 dihydrochloride (ab120607), by ICC/IF. Decrease in NR2B expression correlates with increased concentration of GBR 12909 dihydrochloride, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120607 (GBR 12909 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab65783 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Wiskerke J  et al. µ-Opioid receptors in the nucleus accumbens shell region mediate the effects of amphetamine on inhibitory control but not impulsive choice. J Neurosci 31:262-72 (2011). Read more (PubMed: 21209211) »
  • Szasz BK  et al. Nicotinic acetylcholine receptor antagonistic property of the selective dopamine uptake inhibitor, GBR-12909 in rat hippocampal slices. Neuroscience 145:344-9 (2007). Read more (PubMed: 17207584) »
  • Mike A  et al. Inhibitory effect of the DA uptake blocker GBR 12909 on sodium channels of hippocampal neurons. Neuroreport 14:1945-9 (2003). Read more (PubMed: 14561926) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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