Overview

  • Product nameAnti-Gbx2 antibody
    See all Gbx2 primary antibodies
  • Description
    Goat polyclonal to Gbx2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QGKDESKVEDDPK

    , corresponding to internal sequence amino acids 181-193 of Human Gbx2.

  • Positive control
    • Jurkat cell lysate, Human brain (cortex) and Human placenta tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab109726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 37 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 3.75 µg/ml.

Target

Anti-Gbx2 antibody images

  • Anti-Gbx2 antibody (ab109726) at 0.1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 37 kDa
  • Human Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab109726 at a concentration of 3.75 µg/ml.
  • Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab109726 at a concentration of 3.75 µg/ml.

References for Anti-Gbx2 antibody (ab109726)

ab109726 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic Stem Cells)
Permeabilization Yes - 0.2% TritonX
Specification Embryonic Stem Cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde
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Submitted Aug 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"