Overview

  • Product name
  • Description
    Rabbit polyclonal to GC1q R
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Chimpanzee, Gorilla, Orangutan, Bat
  • Immunogen

    Immunogen corresponds to a region within amino acids 150-200 of Human GC1q R (NP_001203.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cells lysate IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab101267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 31 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Binds to the globular "heads" of C1Q thus inhibiting C1 activation.
  • Sequence similarities
    Belongs to the MAM33 family.
  • Cellular localization
    Mitochondrion matrix. Nucleus. Might also be nuclear in some cell types.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASF/SF2 associated protein p32 antibody
    • C1q globular domain binding protein antibody
    • C1qBP antibody
    • C1QBP_HUMAN antibody
    • Complement component 1 q subcomponent binding protein antibody
    • Complement component 1 Q subcomponent binding protein mitochondrial antibody
    • Complement component 1 Q subcomponent-binding protein, mitochondrial antibody
    • GC1Q R antibody
    • GC1q R protein antibody
    • GC1q-R protein antibody
    • GC1QBP antibody
    • GC1QR antibody
    • globular domain of, C1q, receptor for antibody
    • Glycoprotein gC1qBP antibody
    • HABP 1 antibody
    • HABP1 antibody
    • Hyaluronan binding protein 1 antibody
    • Hyaluronan-binding protein 1 antibody
    • Mitochondrial matrix protein p32 antibody
    • p32 antibody
    • p32 splicing factor antibody
    • p33 antibody
    • Pre mrna splicing factor SF2 P32 subunit precursor antibody
    • SF2p32 antibody
    • Splicing factor SF2 associated protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling GC1q R with ab101267 at 1/1000 (1µg/ml).
  • ICC/IF image of ab101267 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101267, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GC1q R antibody (ab101267) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : 293T whole cell lysate at 50 µg/ml
    Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 31 kDa


    Exposure time : 30 seconds
  • Detection of Human GC1q R in 1mg HeLa whole cell lysate (20% of IP loaded) using ab101267 at 3µg/mg of lysate. Further Western Blot detection was performed using ab101267 at 0.4µg/ml.
    Lane 1: ab101267 IP.
    Lane 2: control IgG IP.

References

ab101267 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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