Anti-GCAP1 antibody [G2] (ab5420)

Overview

  • Product name
    Anti-GCAP1 antibody [G2]
    See all GCAP1 primary antibodies
  • Description
    Mouse monoclonal [G2] to GCAP1
  • Specificity
    This antibody is specific for GCAP 1 and does not react with other isotypes.
  • Tested applications
    Suitable for: IP, Inhibition Assay, WBmore details
  • Species reactivity
    Reacts with: Cow, Human, Non human primates
  • Immunogen

    Other Immunogen Type corresponding to GCAP1. Bacterial expressed full length GCAP1.

  • Positive control
    • WB: bovine rod outer segment membranes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP 1 and GCAP 2. It has been demonstrated that both GCAP 1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP 1 (GenBank AF172707) is predominantly localized in the photoreceptor outer segments, while GCAP 2 is found in retina samples.
  • Clonality
    Monoclonal
  • Clone number
    G2
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa.

Target

  • Function
    Stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.
  • Tissue specificity
    Retina; cone outer and inner segments, in particular, in disk membrane regions, and to a lesser extent rod inner and outer segments.
  • Involvement in disease
    Defects in GUCA1A are the cause of cone dystrophy type 3 (COD3) [MIM:602093]. COD3 is an autosomal dominant cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies, in which some loss of peripheral vision also occurs.
  • Sequence similarities
    Contains 4 EF-hand domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COD3 antibody
    • GCAP 1 antibody
    • GCAP antibody
    • Guanylate Cyclase Activating Protein Photoreceptor 1 antibody
    • Guanylate cyclase activator 1A antibody
    • Guanylin 1 antibody
    • guanylyl cyclase activating protein 1 antibody
    • Guanylyl cyclase-activating protein 1 antibody
    • GUC1A_HUMAN antibody
    • GUCA antibody
    • GUCA1 antibody
    • GUCA1A antibody
    see all

Anti-GCAP1 antibody [G2] images

  • Western blot of GCAP 1 on bovine rod outer segment lysate using ab5420.

References for Anti-GCAP1 antibody [G2] (ab5420)

ab5420 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Both of these antibodies have been made against the full length GCAP1 and full length GCAP2 proteins. These antibodies have not been epitope mapped, and therefore we do not know the exact peptide sequence that the anti-GCAP...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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