Anti-GCAP2 antibody [A1] (ab5422)

Overview

  • Product name
    Anti-GCAP2 antibody [A1]
    See all GCAP2 primary antibodies
  • Description
    Mouse monoclonal [A1] to GCAP2
  • Specificity
    This antibody is specific for GCAP 2 and does not react with other isotypes.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Other Immunogen Type corresponding to GCAP2. Bacterial expressed full length GCAP2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP 1 and GCAP 2. It has been demonstrated that both GCAP 1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP-1 is predominantly localized in the photoreceptor outer segments, while GCAP 2 is found in retina samples.
  • Clonality
    Monoclonal
  • Clone number
    A1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa.

Target

  • Function
    Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.
  • Tissue specificity
    Retina. Cones and rod.
  • Sequence similarities
    Contains 4 EF-hand domains.
  • Cellular localization
    Cell membrane. Membranes of outer segment.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCAP 2 antibody
    • Guanylate Cyclase Activating Protein Photoreceptor 2 antibody
    • Guanylate cyclase activator 1B antibody
    • Guanylin 2 antibody
    • Guanylyl cyclase-activating protein 2 antibody
    • GUC1B_HUMAN antibody
    • GUCA 2 antibody
    • Guca1b antibody
    see all

References for Anti-GCAP2 antibody [A1] (ab5422)

ab5422 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Both of these antibodies have been made against the full length GCAP1 and full length GCAP2 proteins. These antibodies have not been epitope mapped, and therefore we do not know the exact peptide sequence that the anti-GCAP...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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