Overview

  • Product name
  • Description
    Rabbit polyclonal to GCAT
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, from the internal region of Human GCAT.

  • Positive control
    • Mouse liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA 1/1000.

Target

  • Tissue specificity
    Strongly expressed in heart, brain, liver and pancreas. Also found in lung.
  • Pathway
    Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 2/2.
  • Sequence similarities
    Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 amino 3 ketobutyrate CoA ligase antibody
    • 2 amino 3 ketobutyrate coenzyme A ligase, mitochondrial antibody
    • 2-amino-3-ketobutyrate coenzyme A ligase antibody
    • AKB ligase antibody
    • Aminoacetone synthase antibody
    • GCAT antibody
    • Glycine acetyltransferase antibody
    • glycine C acetyltransferase antibody
    • glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase) antibody
    • KBL_HUMAN antibody
    • KLB antibody
    • mitochondrial antibody
    see all

Images

  • Anti-GCAT antibody (ab85202) at 1/50 dilution + Mouse liver tissue lysates at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 32 kDa,65 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Kim D  et al. SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance. Nature 520:363-7 (2015). Read more (PubMed: 25855294) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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