Recombinant
RabMAb

Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

Overview

  • Product name
    Anti-GCDFP 15 antibody [EP1582Y]
    See all GCDFP 15 primary antibodies
  • Description
    Rabbit monoclonal [EP1582Y] to GCDFP 15
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human GCDFP 15

  • Positive control
    • T47D cell lysate, human breast carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 17 kDa.Can be blocked with GCDFP 15 peptide (ab155648).
IHC-P 1/100 - 1/250.
ICC 1/50 - 1/100.
Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity
      Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
    • Sequence similarities
      Belongs to the PIP family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRST-2 antibody
      • BRST2 antibody
      • GCDFP-15 antibody
      • GCDGP15 antibody
      • gp17 antibody
      • GPIP4 antibody
      • Gross cystic disease fluid protein 15 antibody
      • PIP antibody
      • PIP_HUMAN antibody
      • Prolactin induced protein antibody
      • Prolactin inducible protein precursor antibody
      • Prolactin-induced protein antibody
      • Prolactin-inducible protein antibody
      • SABP antibody
      • Secretory actin binding protein antibody
      • Secretory actin-binding protein antibody
      see all

    Anti-GCDFP 15 antibody [EP1582Y] images

    • Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution + T47D cell lysate at 10 µg

      Secondary
      goat anti rabbit HRP at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120   AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

      For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.  

      Confocal image showing cytoplasmic staining on MCF7 cells.

    • Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363
    • ab62363 showing positive staining in Cystic breast disease tissue.

    • Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).

    • ab62363 showing positive staining in Salivary gland tissue.

    • ab62363 showing negative staining in Hepatocellular carcinoma tissue.

    • ab62363 showing negative staining in Prostate hyperplasia tissue.

    References for Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

    This product has been referenced in:
    • Guo Z  et al. Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification. J Transl Med 13:371 (2015). WB . Read more (PubMed: 26608305) »
    • Gallo A  et al. Gross Cystic Disease Fluid Protein-15(GCDFP-15)/Prolactin-Inducible Protein (PIP) as Functional Salivary Biomarker for Primary Sjögren's Syndrome. J Genet Syndr Gene Ther 4:N/A (2013). IHC . Read more (PubMed: 24416635) »

    See all 4 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Methanol
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 28 2014

    The antibody ab62363 has an immunogen near the N-terminus of human GCDFP 15. The peptide ab48069 you mention however is derived from the internal region of GCDFP 15 and therefore ab62363 will not bind to it.

    I have been in contact with the la...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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