Recombinant Anti-GCDFP 15 antibody [EP1582Y] (ab62363)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1582Y] to GCDFP 15
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GCDFP 15 antibody [EP1582Y]
See all GCDFP 15 primary antibodies -
Description
Rabbit monoclonal [EP1582Y] to GCDFP 15 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- T47D cell lysate, human breast carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1582Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF | (1) |
1/50 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Predicted molecular weight: 17 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/50 - 1/100. |
Target
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Tissue specificity
Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands. -
Sequence similarities
Belongs to the PIP family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5304 Human
- Omim: 176720 Human
- SwissProt: P12273 Human
- Unigene: 99949 Human
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Alternative names
- BRST-2 antibody
- BRST2 antibody
- GCDFP-15 antibody
see all
Images
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Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution + T47D cell lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).
For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.
Confocal image showing cytoplasmic staining on MCF7 cells.
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Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363
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ab62363 showing positive staining in Cystic breast disease tissue.
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Intracellular Flow Cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).
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ab62363 showing positive staining in Salivary gland tissue.
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ab62363 showing negative staining in Hepatocellular carcinoma tissue.
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ab62363 showing negative staining in Prostate hyperplasia tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab62363 has been referenced in 6 publications.
- Costa-da-Silva AC et al. Salivary ZG16B expression loss follows exocrine gland dysfunction related to oral chronic graft-versus-host disease. iScience 25:103592 (2022). PubMed: 35005541
- Hanamura T et al. Secreted indicators of androgen receptor activity in breast cancer pre-clinical models. Breast Cancer Res 23:102 (2021). PubMed: 34736512
- Guo Z et al. Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification. J Transl Med 13:371 (2015). WB . PubMed: 26608305
- Gallo A et al. Gross Cystic Disease Fluid Protein-15(GCDFP-15)/Prolactin-Inducible Protein (PIP) as Functional Salivary Biomarker for Primary Sjögren's Syndrome. J Genet Syndr Gene Ther 4:N/A (2013). IHC . PubMed: 24416635
- Naderi A & Meyer M Prolactin-induced protein mediates cell invasion and regulates integrin signaling in estrogen receptor-negative breast cancer. Breast Cancer Res 14:R111 (2012). WB, IHC-P ; Human . PubMed: 22817771
- Baniwal SK et al. Runx2 transcriptome of prostate cancer cells: insights into invasiveness and bone metastasis. Mol Cancer 9:258 (2010). WB ; Human . PubMed: 20863401