Overview

  • Product name
  • Description
    Mouse polyclonal to GCDH
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length GCDH protein (Human)

  • Positive control
    • Human liver tissue lysate and 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects bands of approximately 46 and 48 kDa (predicted molecular weight: 48 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
    • Tissue specificity
      Isoform 1 and isoform 2 are expressed in fibroblasts and liver.
    • Pathway
      Amino-acid metabolism; lysine degradation.
      Amino-acid metabolism; tryptophan metabolism.
    • Involvement in disease
      Defects in GCDH are the cause of glutaric aciduria type 1 (GA1) [MIM:231670]. GA1 is an autosomal recessive metabolic disorder characterized by progressive dystonia and athetosis due to gliosis and neuronal loss in the basal ganglia.
    • Sequence similarities
      Belongs to the acyl-CoA dehydrogenase family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAD5 antibody
      • EC 1.3.99.7 antibody
      • GCD antibody
      • Gcdh antibody
      • GCDH_HUMAN antibody
      • Glutaryl CoA dehydrogenase antibody
      • Glutaryl CoA dehydrogenase, mitochondrial antibody
      • Glutaryl Coenzyme A dehydrogenase antibody
      • Glutaryl-CoA dehydrogenase antibody
      • mitochondrial antibody
      • MS781 antibody
      see all

    Anti-GCDH antibody images

    • Anti-GCDH antibody (ab67103) at 1/500 dilution + human liver tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa
    • All lanes : Anti-GCDH antibody (ab67103) at 1/500 dilution

      Lane 1 : GCDH transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa
      Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-GCDH antibody (ab67103)

    ab67103 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67103.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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