Overview

  • Product name
  • Description
    Rabbit polyclonal to GCET1
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GCET1 aa 201-230 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q86WD7

  • Positive control
    • Human lymph tissue; MCF-7 cell lysate; MDA-231 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab176176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 47 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).
  • Tissue specificity
    Highly expressed in normal germinal center (GC) B-cells and GC B-cell-derived malignancies.
  • Sequence similarities
    Belongs to the serpin family.
  • Cellular localization
    Cytoplasm; Secreted and Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centerin antibody
    • GCET1 antibody
    • Germinal center B cell expressed transcript 1 antibody
    • Germinal center B-cell-expressed transcript 1 protein antibody
    • Seprin A9 antibody
    • Serine proteinase inhibitor A11 antibody
    • Serpin A9 antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 9 antibody
    • Serpin peptidase inhibitor clade A member 9 antibody
    • SERPINA11 antibody
    • SERPINA11b antibody
    • Serpina9 antibody
    • SPA9_HUMAN antibody
    see all

Images

  • Anti-GCET1 antibody (ab176176) at 1/1000 dilution + MCF-7 cell lysate at 35 µg

    Predicted band size : 47 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lymph tissue labeling GCET1 with ab176176 at 1/10 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Flow cytometric analysis of MDA-231 cells labelling GCET1 using ab176176 (bottom histogram) or negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab176176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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